Identification of a molecular signature that influences neutrophil migration and function in urinary tract infection during chronic lymphocytic leukaemia

Neutrophils are very important leukocytes for the acute control of urinary tract infection (UTI). However, patients that suffer from chronic lymphocytic leukaemia (CLL) are highly susceptible to bacterial infection, such as UTI. Here, we will use models that combine CLL and UTI and will evaluate molecules that affect neutrophil migration and function and predispose to severe bacterial infections in CLL.

Engel
Daniel Robert, PhD

UDE

Institute for Experimental Immunology and Imaging

Team

Shevchuk
Olga, PostDoc

UDE

Widera
Lars Christian, PhD student

UDE

Publications

A3 Z1 A Reference Database Enabling In-Depth Proteome and PTM Analysis of Mouse Immune Cells Siemes, D.; Voss, H.; Benvenuti, F.; Simoncello, F.; Kopczynski, D.; Siebels, B.; Schlüter, H.; Kollipara, L.; Sickmann, A.; Engel, D. R.; Shevchuk, O. 2025 Sci Data https://doi.org/10.1038/s41597-025-04829-9
A3 Z1 C6 Inhibition of Tumour Necrosis Factor Alpha by Etanercept Attenuates Shiga Toxin-Induced Brain Pathology Christ, R.; Siemes, D.; Zhao, S.; Widera, L.; Spangenberg, P.; Lill, J.; Thiebes, S.; Bottek, J.; Borgards, L.; Pinho, A. G.; Silva, N. A.; Monteiro, S.; Jorch, S. K.; Gunzer, M.; Siebels, B.; Voss, H.; Schlüter, H.; Shevchuk, O.; Chen, J.; Engel, D. R. 2025 J Neuroinflammation https://doi.org/10.1186/s12974-025-03356-z
Z1 A2 A3 C6 msiFlow: automated workflows for reproducible and scalable multimodal mass spectrometry imaging and microscopy data analysis Spangenberg, P.; Bessler, S.; Widera, L.; Bottek, J.; Richter, M.; Thiebes, S.; Siemes, D.; Krauß, S. D.; Migas, L. G.; Kasarla, S. S.; Phapale, P.; Kleesiek, J.; Führer, D.; Moeller, L. C.; Heuer, H.; Van de Plas, R.; Gunzer, M.; Soehnlein, O.; Soltwisch, J.; Shevchuk, O.; Dreisewerd, K.; Engel, D. R. 2025 Nat Commun https://doi.org/10.1038/s41467-024-55306-7
C6 A2 A3 Z1 C5 Stroke and Myocardial Infarction Induce Neutrophil Extracellular Trap Release Disrupting Lymphoid Organ Structure and Immunoglobulin Secretion Tuz, A. A.; Ghosh, S.; Karsch, L.; Ttoouli, D.; Sata, S. P.; Ulusoy, Ö.; Kraus, A.; Hoerenbaum, N.; Wolf, J.-N.; Lohmann, S.; Zwirnlein, F.; Kaygusuz, V.; Lakovic, V.; Tummes, H.-L.; Beer, A.; Gallert, M.; Thiebes, S.; Qefalia, A.; Cibir, Z.; Antler, M.; Korste, S.; Haj Yehia, E.; Michel, L.; Rassaf, T.; Kaltwasser, B.; Abdelrahman, H.; Mohamud Yusuf, A.; Wang, C.; Yin, D.; Haeusler, L.; Lueong, S.; Richter, M.; Engel, D. R.; Stenzel, M.; Soehnlein, O.; Frank, B.; Solo-Nomenjanahary, M.; Ho-Tin-Noé, B.; Siveke, J. T.; Totzeck, M.; Hoffmann, D.; Grüneboom, A.; Hagemann, N.; Hasenberg, A.; Desilles, J.-P.; Mazighi, M.; Sickmann, A.; Chen, J.; Hermann, D. M.; Gunzer, M.; Singh, V. 2024 Nat Cardiovasc Res https://doi.org/10.1038/s44161-024-00462-8
A3 Z1 Scientific Advances in Understanding the Pathogenesis, Diagnosis, and Prevention of Urinary Tract Infection in the Past 10 Years Engel, D. R.; Wagenlehner, F. M. E.; Shevchuk, O. 2024 Infect Dis Clin North Am https://doi.org/10.1016/j.idc.2024.03.002
A2 A3 A4 A5 A6 A7 B1 B2 B3 B5 C1 C2 C3 C5 AP1 Neutrophils - Biology and Diversity Maier-Begandt, D.; Alonso-Gonzalez, N.; Klotz, L.; Erpenbeck, L.; Jablonska, J.; Immler, R.; Hasenberg, A.; Mueller, T. T.; Herrero-Cervera, A.; Aranda-Pardos, I.; Flora, K.; Zarbock, A.; Brandau, S.; Schulz, C.; Soehnlein, O.; Steiger, S.; the TRR332 consortium. 2023 Nephrology Dialysis Transplantation https://doi.org/10.1093/ndt/gfad266
A3 A5 Z1 Comparative transcriptomic and proteomic signature of lung alveolar macrophages reveals the integrin CD11b as a regulatory hub during pneumococcal pneumonia infection Zec, K.; Thiebes, S.; Bottek, J.; Siemes, D.; Spangenberg, P.; Trieu, D. V.; Kirstein, N.; Subramaniam, N.; Christ, R.; Klein, D.; Jendrossek, V.; Loose, M.; Wagenlehner, F.; Jablonska, J.; Bracht, T.; Sitek, B.; Budeus, B.; Klein-Hitpass, L.; Theegarten, D.; Shevchuk, O.; Engel, D. R. 2023 Front. Immunol. https://doi.org/10.3389/fimmu.2023.1227191
A3 C5 C6 Z1 Rapid and Fully Automated Blood Vasculature Analysis in 3D Light-Sheet Image Volumes of Different Organs Spangenberg, P.; Hagemann, N.; Squire, A.; Förster, N.; Krauß, S. D.; Qi, Y.; Yusuf, A. M.; Wang, J.; Grüneboom, A.; Kowitz, L.; Korste, S.; Totzeck, M.; Cibir, Z.; Tuz, A. A.; Singh, V.; Siemes, D.; Struensee, L.; Engel, D. R.; Ludewig, P.; Nascentes Melo, L. M.; Helfrich, I.; Chen, J.; Gunzer, M.; Hermann, D. M.; Mosig, A. 2023 Cell Reports Methods https://doi.org/10.1016/j.crmeth.2023.100436
A3 Non-Invasive Mapping of Systemic Neutrophil Dynamics upon Cardiovascular Injury Bouvain, P.; Ding, Z.; Kadir, S.; Kleimann, P.; Kluge, N.; Tiren, Z.-B.; Steckel, B.; Flocke, V.; Zalfen, R.; Petzsch, P.; Wachtmeister, T.; John, G.; Subramaniam, N.; Krämer, W.; Strasdeit, T.; Mehrabipour, M.; Moll, J. M.; Schubert, R.; Ahmadian, M. R.; Bönner, F.; Boeken, U.; Westenfeld, R.; Engel, D. R.; Kelm, M.; Schrader, J.; Köhrer, K.; Grandoch, M.; Temme, S.; Flögel, U. 2023 Nature Cardiovascular Research https://www.nature.com/articles/s44161-022-00210-w