The role of KV1.3 on Ca2+ signalling and neutrophil effector functions

Neutrophils play a critical role during the inflammatory response. Recent studies have demonstrated that loss of the voltage-gated potassium channel KV1.3 impairs sustained calcium signalling during neutrophil activation leading to impaired phagocytosis and neutrophil adhesion. Here we aim to dissect the exact role of KV1.3 in neutrophil functionality with specific focus on neutrophil recruitment, NET release and inflammasome activation.

Sperandio
Markus, MD

LMU

Institute of Cardiovascular Physiology and Pathophysiology

Team

Immler
Roland, PostDoc

LMU

Publications

C2 C3 B5 C7 Cytosolic S100A8/A9 Promotes Ca2+ Supply at LFA-1 Adhesion Clusters during Neutrophil Recruitment Napoli, M.; Immler, R.; Rohwedder, I.; Lupperger, V.; Pfabe, J.; Gonzalez Pisfil, M.; Yevtushenko, A.; Vogl, T.; Roth, J.; Salvermoser, M.; Dietzel, S.; Slak Rupnik, M.; Marr, C.; Walzog, B.; Sperandio, M.; Pruenster, M. 2024 Elife https://doi.org/10.7554/eLife.96810
C2 C7 Neonatal neutrophils exhibit reduced NLRP3 inflammasome activation Wackerbarth, L. M.; Seifert, S. B.; Napoli, M.; Rohwedder, I.; Vogl, T.; Scheiermann, C.; Kolben, T.; Nussbaum, C.; Pruenster, M.; Immler, R.; Sperandio, M. 2024 J Leukoc Biol https://doi.org/10.1093/jleuko/qiae206
C2 CCR3-Dependent Eosinophil Recruitment Is Regulated by Sialyltransferase ST3Gal-IV. Immler, R.; Nussbaumer, K.; Doerner, A.; El Bounkari, O.; Huber, S.; Abisch, J.; Napoli, M.; Schmidt, S.; Margraf, A.; Pruenster, M.; Rohwedder, I.; Lange-Sperandio, B.; Mall, M. A.; de Jong, R.; Ohnmacht, C.; Bernhagen, J.; Voehringer, D.; Marth, J. D.; Frommhold, D.; Sperandio, M. 2024 Proceedings of the National Academy of Sciences https://doi.org/10.1073/pnas.2319057121
C2 Cyclophilin A Is a Ligand for RAGE in Thrombo-Inflammation Seizer, P.; von Ungern-Sternberg, S. N. I.; Haug, V.; Dicenta, V.; Rosa, A.; Butt, E.; Nöthel, M.; Rohlfing, A.-K.; Sigle, M.; Nawroth, P. P.; Nussbaum, C.; Sperandio, M.; Kusch, C.; Meub, M.; Sauer, M.; Münzer, P.; Bieber, K.; Stanger, A.; Mack, A. F.; Huber, R.; Brand, K.; Lehners, M.; Feil, R.; Poso, A.; Krutzke, K.; Schäffer, T. E.; Nieswandt, B.; Borst, O.; May, A. E.; Zernecke, A.; Gawaz, M.; Heinzmann, D. 2024 Cardiovasc Res https://doi.org/10.1093/cvr/cvad189
C2 Circadian Clock and Hypoxia Sartor, F.; Ferrero-Bordera, B.; Haspel, J.; Sperandio, M.; Holloway, P. M.; Merrow, M. 2024 Circulation Research https://doi.org/10.1161/CIRCRESAHA.124.323518
C2 Endothelial TDP-43 controls sprouting angiogenesis and vascular barrier integrity, and its deletion triggers neuroinflammation Arribas, V.; Onetti, Y.; Ramiro-Pareta, M.; Villacampa, P.; Beck, H.; Alberola, M.; Esteve-Codina, A.; Merkel, A.; Sperandio, M.; Martínez-Estrada, O. M.; Schmid, B.; Montanez, E. 2024 JCI Insight https://doi.org/10.1172/jci.insight.177819
A2 A3 A4 A5 A6 A7 B1 B2 B3 B5 C1 C2 C3 C5 AP1 Neutrophils - Biology and Diversity Maier-Begandt, D.; Alonso-Gonzalez, N.; Klotz, L.; Erpenbeck, L.; Jablonska, J.; Immler, R.; Hasenberg, A.; Mueller, T. T.; Herrero-Cervera, A.; Aranda-Pardos, I.; Flora, K.; Zarbock, A.; Brandau, S.; Schulz, C.; Soehnlein, O.; Steiger, S.; the TRR332 consortium. 2023 Nephrology Dialysis Transplantation https://doi.org/10.1093/ndt/gfad266
B5 C2 C7 E-Selectin-Mediated Rapid NLRP3 Inflammasome Activation Regulates S100A8/S100A9 Release from Neutrophils via Transient Gasdermin D Pore Formation. Pruenster, M.; Immler, R.; Roth, J.; Kuchler, T.; Bromberger, T.; Napoli, M.; Nussbaumer, K.; Rohwedder, I.; Wackerbarth, L. M.; Piantoni, C.; Hennis, K.; Fink, D.; Kallabis, S.; Schroll, T.; Masgrau-Alsina, S.; Budke, A.; Liu, W.; Vestweber, D.; Wahl-Schott, C.; Roth, J.; Meissner, F.; Moser, M.; Vogl, T.; Hornung, V.; Broz, P.; Sperandio, M. 2023 Nat Immunol https://doi.org/10.1038/s41590-023-01656-1
A1 A2 C2 Z1 The RNA editor ADAR2 promotes immune cell trafficking by enhancing endothelial responses to interleukin-6 during sterile inflammation Gatsiou, A.; Tual-Chalot, S.; Napoli, M.; Ortega-Gomez, A.; Regen, T.; Badolia, R.; Cesarini, V.; Garcia-Gonzalez, C.; Chevre, R.; Ciliberti, G.; Silvestre-Roig, C.; Martini, M.; Hoffmann, J.; Hamouche, R.; Visker, J. R.; Diakos, N.; Wietelmann, A.; Silvestris, D. A.; Georgiopoulos, G.; Moshfegh, A.; Schneider, A.; Chen, W.; Guenther, S.; Backs, J.; Kwak, S.; Selzman, C. H.; Stamatelopoulos, K.; Rose-John, S.; Trautwein, C.; Spyridopoulos, I.; Braun, T.; Waisman, A.; Gallo, A.; Drakos, S. G.; Dimmeler, S.; Sperandio, M.; Soehnlein, O.; Stellos, K. 2023 Immunity https://doi.org/10.1016/j.immuni.2023.03.021
A6 C2 C3 C6 A20 and the Noncanonical NF-κB Pathway Are Key Regulators of Neutrophil Recruitment during Fetal Ontogeny Rohwedder, I.; Wackerbarth, L. M.; Heinig, K.; Ballweg, A.; Altstätter, J.; Ripphahn, M.; Nussbaum, C.; Salvermoser, M.; Bierschenk, S.; Straub, T.; Gunzer, M.; Schmidt-Supprian, M.; Kolben, T.; Schulz, C.; Ma, A.; Walzog, B.; Heinig, M.; Sperandio, M. 2023 JCI Insight https://doi.org/10.1172/jci.insight.155968
B5 C2 C7 Complex Regulation of Alarmins S100A8/A9 and Secretion via Gasdermin-D Pores Exacerbates Autoinflammation in Familial Mediterranean Fever Jorch, S. K.; McNally, A.; Berger, P.; Wolf, J.; Kaiser, K.; Chetrusca Covash, A.; Robeck, S.; Pastau, I.; Fehler, O.; Jauch-Speer, S.-L.; Hermann, S.; Schäfers, M.; Van Gorp, H.; Kanneganti, A.; Dehoorne, J.; Haerynck, F.; Penco, F.; Gattorno, M.; Chae, J. J.; Kubes, P.; Lamkanfi, M.; Wullaert, A.; Sperandio, M.; Vogl, T.; Roth, J.; Austermann, J. 2023 J Allergy Clin Immunol https://doi.org/10.1016/j.jaci.2023.01.037