The role of Ly6g and CD177 as “eat me” signals to control neutrophil-mediated tissue destruction in stroke DE
Among murine leukocytes neutrophils exclusively express high levels of Ly6G. The human analogue, CD177, is lacking on neutrophils in ~5% of the general population. However, the functional role of both molecules remains unknown. In this project, we will investigate the hypothesis that Ly6G/CD177mediates the phagocytosis of brain-infiltrating neutrophils through microglia. By elucidating the underlying mechanisms, we aim to identify means for its selective therapeutic modulation in stroke. In a clinical setting we will investigate the importance of CD177 for the outcome and future clinical management of human stroke.
Team
Publications
Proteomic Characterization of 1,000 Human and Murine Neutrophils Freshly Isolated from Blood and Sites of Sterile Inflammation.Ghosh, S.; Tuz, A. A.; Stenzel, M.; Singh, V.; Richter, M.; Soehnlein, O.; Lange, E.; Heyer, R.; Cibir, Z.; Beer, A.; Jung, M.; Nagel, D.; Hermann, D. M.; Hasenberg, A.; Grüneboom, A.; Sickmann, A.; Gunzer, M.
2024 Mol Cell Proteomics
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Extracellular vesicles set the stage for brain plasticity and recovery by multimodal signallingHermann, D. M.; Peruzzotti-Jametti, L.; Giebel, B.; Pluchino, S.
2024 Brain
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Arterial Specification Precedes Microvascular Restitution in the Peri-Infarct Cortex That Is Driven by Small MicrovesselsHagemann, N.; Qi, Y.; Mohamud Yusuf, A.; Li, A.; Zhang, X.; Spangenberg, P.; Squire, A.; Doeppner, T. R.; Jin, F.; Zhao, S.; Chen, J.; Mosig, A.; Gunzer, M.; Hermann, D. M. .
2024 J Cereb Blood Flow Metab
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Stroke and Myocardial Infarction Induce Neutrophil Extracellular Trap Release Disrupting Lymphoid Organ Structure and Immunoglobulin SecretionTuz, A. A.; Ghosh, S.; Karsch, L.; Ttoouli, D.; Sata, S. P.; Ulusoy, Ö.; Kraus, A.; Hoerenbaum, N.; Wolf, J.-N.; Lohmann, S.; Zwirnlein, F.; Kaygusuz, V.; Lakovic, V.; Tummes, H.-L.; Beer, A.; Gallert, M.; Thiebes, S.; Qefalia, A.; Cibir, Z.; Antler, M.; Korste, S.; Haj Yehia, E.; Michel, L.; Rassaf, T.; Kaltwasser, B.; Abdelrahman, H.; Mohamud Yusuf, A.; Wang, C.; Yin, D.; Haeusler, L.; Lueong, S.; Richter, M.; Engel, D. R.; Stenzel, M.; Soehnlein, O.; Frank, B.; Solo-Nomenjanahary, M.; Ho-Tin-Noé, B.; Siveke, J. T.; Totzeck, M.; Hoffmann, D.; Grüneboom, A.; Hagemann, N.; Hasenberg, A.; Desilles, J.-P.; Mazighi, M.; Sickmann, A.; Chen, J.; Hermann, D. M.; Gunzer, M.; Singh, V.
2024 Nat Cardiovasc Res
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ComplexEye: a multi-lens array microscope for high-throughput cell migration analysis |
Microvascular Network Remodeling in the Ischemic Mouse Brain Defined by Light Sheet MicroscopyNina Hagemann, Yachao Qi, Ayan Mohamud Yusuf, AnRan Li, Anthony Squire, Tobias Tertel, Bernd Giebel, Peter Ludewig, Philippa Spangenberg, Jianxu Chen, Axel Mosig, Matthias Gunzer and Dirk M. Hermann
2024 Arteriosclerosis, Thrombosis, and Vascular Biology
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Modulating microglia to enhance neuroplasticity for restoring brain function after strokeLiu, Z.; Hermann, D. M.; Dzyubenko, E.; Cao, G.; Cao, X.
2023 Front. Cell. Neurosci.
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Structural changes in perineuronal nets and their perforating GABAergic synapses precede motor coordination recovery post strokeDzyubenko, E.; Willig, K. I.; Yin, D.; Sardari, M.; Tokmak, E.; Labus, P.; Schmermund, B.; Hermann, D. M.
2023 Journal of Biomedical Science
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Neural precursor cell delivery induces acute post-ischemic cerebroprotection, but fails to promote long-term stroke recovery in hyperlipidemic mice due to mechanisms that include pro-inflammatory responses associated with brain hemorrhagesYin, D.; Wang, C.; Qi, Y.; Wang, Y.-C.; Hagemann, N.; Mohamud Yusuf, A.; Dzyubenko, E.; Kaltwasser, B.; Tertel, T.; Giebel, B.; Gunzer, M.; Popa-Wagner, A.; Doeppner, T. R.; Hermann, D. M.
2023 J Neuroinflammation
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ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysisCibir, Z.; Hassel, J.; Sonneck, J.; Kowitz, L.; Beer, A.; Kraus, A.; Hallekamp, G.; Rosenkranz, M.; Raffelberg, P.; Olfen, S.; Smilowski, K.; Burkard, R.; Helfrich, I.; Tuz, A. A.; Singh, V.; Ghosh, S.; Sickmann, A.; Klebl, A.-K.; Eickhoff, J. E.; Klebl, B.; Seidl, K.; Chen, J.; Grabmaier, A.; Viga, R.; Gunzer, M.
2023 Nature Communications
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Externalized Histones Fuel Pulmonary Fibrosis via a Platelet-Macrophage Circuit of TGFβ1 and IL-27Riehl, D. R.; Sharma, A.; Roewe, J.; Murke, F.; Ruppert, C.; Eming, S. A.; Bopp, T.; Kleinert, H.; Radsak, M. P.; Colucci, G.; Subramaniam, S.; Reinhardt, C.; Giebel, B.; Prinz, I.; Guenther, A.; Strand, D.; Gunzer, M.; Waisman, A.; Ward, P. A.; Ruf, W.; Schäfer, K.; Bosmann, M.
2023 Proceedings of the National Academy of Sciences
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Rapid and Fully Automated Blood Vasculature Analysis in 3D Light-Sheet Image Volumes of Different OrgansSpangenberg, P.; Hagemann, N.; Squire, A.; Förster, N.; Krauß, S. D.; Qi, Y.; Yusuf, A. M.; Wang, J.; Grüneboom, A.; Kowitz, L.; Korste, S.; Totzeck, M.; Cibir, Z.; Tuz, A. A.; Singh, V.; Siemes, D.; Struensee, L.; Engel, D. R.; Ludewig, P.; Nascentes Melo, L. M.; Helfrich, I.; Chen, J.; Gunzer, M.; Hermann, D. M.; Mosig, A.
2023 Cell Reports Methods
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A20 and the Noncanonical NF-κB Pathway Are Key Regulators of Neutrophil Recruitment during Fetal OntogenyRohwedder, I.; Wackerbarth, L. M.; Heinig, K.; Ballweg, A.; Altstätter, J.; Ripphahn, M.; Nussbaum, C.; Salvermoser, M.; Bierschenk, S.; Straub, T.; Gunzer, M.; Schmidt-Supprian, M.; Kolben, T.; Schulz, C.; Ma, A.; Walzog, B.; Heinig, M.; Sperandio, M.
2023 JCI Insight
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During Early Stages of Cancer, Neutrophils Initiate Anti-Tumor Immune Responses in Tumor-Draining Lymph NodesPylaeva, E.; Korschunow, G.; Spyra, I.; Bordbari, S.; Siakaeva, E.; Ozel, I.; Domnich, M.; Squire, A.; Hasenberg, A.; Thangavelu, K.; Hussain, T.; Goetz, M.; Lang, K. S.; Gunzer, M.; Hansen, W.; Buer, J.; Bankfalvi, A.; Lang, S.; Jablonska, J.
2022 Cell Reports
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Pathological neutrophil migration predicts adverse outcomes in hospitalized patients with liver cirrhosisLanger, M.-M.; Sichelschmidt, S.; Bauschen, A.; Bornemann, L.; Guckenbiehl, S.; Gunzer, M.; Lange, C. M.
2022 Liver International
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Ischemic stroke and concomitant gastrointestinal complications- a fatal combination for patient recoveryTuz, A. A.; Hasenberg, A.; Hermann, D. M.; Gunzer, M.; Singh, V.
2022 Frontiers in Immunology
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